Peptide Therapy: TRIO, The Fountain of Youth for your Skin


Next in our Peptide Series, we take a look at a new peptide combination called Trio. Like other peptides, it can provide a novel remedy to health issues. Trio is like the Fountain of Youth for Skin. It has even been called “natural botox” for some. And, it is topical, so unlike botox, it does not require injections. Please read on for the details!

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We have recently written many blogs about peptides. To recap, a peptide is a small protein, made up of a chain of amino acids. They can be used therapeutically to help with different health conditions. Click on the links to see our articles on peptide therapyHGHCJC 1295/IpamorelinBPC 157PT 141Ta1VIP, Thymosin Beta 4 and KPV.

As we age, our skin changes. Wrinkles, crow’s feet, sun spots, damage to elastic and collagen fibers and other skin changes happen with age (Park J, 2020). Different peptides have been developed to improve skin’s appearance, which are used in skin care products (Park J, 2020).

Could Trio be the Fountain of Youth for your Skin?

Trio is a cosmetic cream that decreases the effects of aging on the skin. It is a synergistic combination of three different peptides: Leuphasyl, Argireline, and GHK-Cu. Trio is made by Tailor Made Health, a supplements company using peptide therapy. We use a number of their products in our clinic. Tailor Made Health makes solutions for anti-aging, muscle repair and growth and anxiety relief. Their Trio skin cream ‘reduces depth of wrinkles, stimulates collagen and has anti-inflammatory effects’ (Tailor Made Health, 2021). Trio is made of (Tailor Made Health, 2021):

  • Leuphasyl is the trademarked name for the peptide Pentapeptide-18
  • Argireline is the trademarked name for the peptide Acetyl Hexapeptide-8.
  • GHK-Cu is a naturally occurring copper peptide found throughout the human body.

What does the Scientific Research Say About these Three Peptides?

Leuphasyl or Pentapeptide-18

Leuphasyl (pentapeptide-18) is a cosmetic peptide that acts as a neurotransmitter inhibitor (Park J, 2020). This gives it Botox-like effects such as reducing fine lines and wrinkles, and improving firmness (Park J, 2020). Leuphasyl is a little less effective than Botox injections, but it lessens wrinkles while still preserving facial expressions (Dragomirescu AO, 2014).

Combining Leuphasyl with Argireline has a synergistic effect, with better results (Dragomirescu AO, 2014). Leuplasyl and Argireline together work better at reducing forehead wrinkles compared to periorbital wrinkles or crow’s feet (Dragomirescu AO, 2014).

One study with Leuphasyl and Argireline showed that it is more effective in young people. The skin improvement is more visible and faster than in mature people (Dragomirescu AO, 2014). This is thought to be because young skin can regenerate more easily (Dragomirescu AO, 2014).

Argireline or Acetyl Hexapeptide-8

Argireline is another Botox-like cosmetic peptide. It is used for collagen stimulation, wound healing, wrinkle smoothing and whitening effects (Park J, 2020). It is effective in reducing wrinkles, by up to 48% with 4 weeks of twice daily treatment (Lim SH, 2018). It induces relaxation of facial muscles similar to Botox, but without any risk of toxicity (Palmieri B, 2020).

In one study, 10% Acetyl hexapeptide‐3 and placebo creams were applied twice daily on 10 women. Argireline resulted in a nearly 30% improvement in crow’s feet wrinkles around the eyes after 30 days, vs. 10% improvement from the placebo (Gorouhi F, 2009).

Another trial compared cream containing 5% Leuphasyl against a cream containing 5% Argireline and a cream with a combination of the two (Gorouhi F, 2009). Wrinkles decreased by 11.64% with Leuphasyl, 16.26% with Argireline and 24.62% with the combination of the two (Gorouhi F, 2009).


GHK-Cu is able to (Pickart L, 2018):

  • Increase collagen synthesis
  • Tighten loose skin and reverse thinning of aged skin
  • Repair protective skin barrier proteins
  • Improve skin firmness, elasticity, and clarity
  • Reduce fine lines, depth of wrinkles and improve structure of aged skin
  • Smooth rough skin
  • Reduce photodamage, mottled hyperpigmentation, skin spots and lesions
  • Improve overall skin appearance
  • Stimulate wound healing through tissue remodelling
  • Protect skin cells from UV radiation
  • Reduce inflammation and free radical damage
  • Down-regulate the expression of metastatic genes, giving it an anti-cancer effect
  • Impact gene expression for anti-aging

Side Effects

Leuphasyl is free from side effects (Dragomirescu AO, 2014). Acetyl hexapeptide-8 did not show any particular adverse effects (Palmieri B, 2020). GHK-Cu is safe, inexpensive and extensively studied (Pickart L, 2018).

The synergistic combination of Leuphasyl, Argireline and GHK-Cu is also safe and effective for slowing and even reversing wrinkles (Tailor Made Health, 2021).

There is always the chance of potential interactions with other medications a person may be taking. It is very important to work with an experienced Functional Medicine practitioner when taking peptide combinations. Our clinic has extensive experience with peptides and can help you.

**  Please stay tuned for our next blog on more Peptide Therapy!  **

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